locus

BSU24610

BSU_24610

geneLength

333

336

outlinks

bsu

BSU24610

BSU_24610

Gene

Coordinates

2,552,653 → 2,552,988

2,552,653 2,552,988

The protein

Paralogous protein(s)

[[protein|SlrR]]

[[this]]

The protein

[SW|Domains]

DNA-binding N-terminal domain (aa 1-69) [Pubmed|21708175]

[[protein|SinI]]-binding C-terminal domain (aa 74-111) [Pubmed|21708175]

DNA-binding N-terminal domain (aa 1-69) [Pubmed|21708175]

[[protein|SinI]]-binding C-terminal domain (aa 74-111) [Pubmed|21708175]

[SW|HTH cro/C1-type domain] (aa 6-61) (according to UniProt)

[SW|Sin domain] (aa 65-103) (according to UniProt)

The protein

additional information

information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000082 PRODORIC2 database]

information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000086 PRODORIC2 database]

Biological materials

Mutant

GP923 (''sinR::spec'') [Pubmed|21856853], available in [SW|Jörg Stülke]'s lab

GP736 (''sinR::tetR'') [Pubmed|24493247], available in [SW|Jörg Stülke]'s lab

1S97 (''sinR''::''phleo''), [Pubmed|8422983], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S97&Search=1S97 BGSC]

GP1672 (''[[gene|sinR]]-[[gene|tasA]]''::''cat'') [Pubmed|24493247], available in [SW|Jörg Stülke]'s lab

BKE24610 (Δ[[gene|sinR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTCATCACCTTCCTTG, downstream forward: _UP4_TAGTGCCTGAGCAGAGGCAC

BKK24610 (Δ[[gene|sinR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTCATCACCTTCCTTG, downstream forward: _UP4_TAGTGCCTGAGCAGAGGCAC

GP923 (''sinR::spec'') [Pubmed|21856853], available in [SW|Jörg Stülke]'s lab

GP736 (''sinR::tetR'') [Pubmed|24493247], available in [SW|Jörg Stülke]'s lab

1S97 (''sinR''::''phleo''), [Pubmed|8422983], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S97&Search=1S97 BGSC]

GP1672 (''[[gene|sinR]]-[[gene|tasA]]''::''cat'') [Pubmed|24493247], available in [SW|Jörg Stülke]'s lab

BKE24610 ([[gene|sinR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTCATCACCTTCCTTG, downstream forward: _UP4_TAGTGCCTGAGCAGAGGCAC

BKK24610 ([[gene|sinR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTCATCACCTTCCTTG, downstream forward: _UP4_TAGTGCCTGAGCAGAGGCAC

Biological materials

Expression vector

N-terminal Strep-tag, for [SW|SPINE], expression in ''B. subtilis'', in [SW|pGP380]: pGP1083 , available in [SW|Jörg Stülke]'s lab

Biological materials

lacZ fusion

pGP1930 (aphA3) based on [[protein|pAC7]], available in [SW|Jörg Stülke]'s lab

pGP1930 (aphA3) based on [SW|pAC7], available in [SW|Jörg Stülke]'s lab

References

Reviews

20395361, 20541494, 23353768, 24988880

20395361, 20541494, 23353768, 24988880, 30218468, 30482826

References

Original publications

22893383, 23378512, 23430750, 23475644, 21856853, 21815947, 22329926, 21326214, 21708175, 8955328, 15661000, 8878039, 24317403, 16923912, 15104138, 16430695, 16430696, 18047568, 18430133, 1906467, 7635837, 11751836, 19201793, 10547280, 9799632, 19788541, 19898538, 3125149, 8932324, 20351052, 20923420, 8422983, 9685500, 9158733, 23646920, 23660663, 24256735, 24347549, 25433524, 26283769, 26819068, 26434553, 28546427

22893383, 23378512, 23430750, 23475644, 21856853, 21815947, 22329926, 21326214, 21708175, 8955328, 15661000, 8878039, 24317403, 16923912, 15104138, 16430695, 16430696, 18047568, 18430133, 1906467, 7635837, 11751836, 19201793, 10547280, 9799632, 19788541, 19898538, 3125149, 8932324, 20351052, 20923420, 8422983, 9685500, 9158733, 23646920, 23660663, 24256735, 24347549, 25433524, 26283769, 26819068, 26434553, 28546427, 29321771, 30181249, 30326845, 30396900, 31493408, 31604312

Biological materials

Expression vectors

N-terminal Strep-tag, for [SW|SPINE], expression in ''B. subtilis'', in [SW|pGP380]: pGP1083 , available in [SW|Jörg Stülke]'s lab

pGP2330: in [SW|pBQ200], for expression in B. subtilis, available in [SW|Jörg Stülke]'s lab